Templates Wizard gallery
H2020 RiskGone - eNanoMapper database
| Template Wizard link | Template | Project/Provider | Type | Category | Status | |
|---|---|---|---|---|---|---|
| COMET | COMET The comet assay, also called SCGE (Single Cell Gel Electrophoresis), is a rapid and informative method to detect DNA damage at single cell level used on many different cell types. The assay detects single and double strand DNA breaks as a consequence of a direct damage or as intermediate of DNA repair processes and it is successfully applied both in in vivo and in vitro genotoxicity testing. | RISKGONE/NILU | doseresponse | Genetic toxicity in vitro | published | |
| CFE | Colony Forming Efficiency The colony forming efficiency assay (CFE) (also called clonogenic or plating efficiency assay) measures the ability of cells to survive and form colonies, which is an ultimate index of cytotoxicity. | RISKGONE/NILU | doseresponse | Cell Viability | published | |
| ALAMARBLUE | Alamar Blue The Alamar Blue (AB) assay is a high throughput, cell metabolism-based method largely applied in toxicology and nanotoxicology to investigate cell viability (cytotoxicity), cell proliferation and cellular metabolic activity in response to chemicals and nanomaterials. | RISKGONE/NILU | doseresponse | Cell Viability | published | |
| WST1 | WST-1 Assay for Cell Proliferation and Viability | GRACIOUS/BfR | doseresponse | Cell Viability | published | |
| NRU | Neutral Red Uptake Assay | GRACIOUS/BfR | doseresponse | Cell Viability | published | |
| HPRT | HPRT gene mutation assay - SWANSEA layout The hypoxanthine-guanine phosphoribosyl transferase (HPRT) gene is located on the X chromosome of mammalian cells and is used as a model gene to investigate gene mutation. The in vitro mammalian cell gene mutation assay is significantly important for detecting point mutations induced by engineered nanomaterials (ENMs) as the bacterial reverse gene mutation assay (Ames test) is not appropriate for use with these materials. | RISKGONE/SWANSEA | doseresponse | Genetic toxicity in vitro | published | |
| HPRTNILU | HPRT gene mutation assay - NILU layout The hypoxanthine-guanine phosphoribosyl transferase (HPRT) gene is located on the X chromosome of mammalian cells and is used as a model gene to investigate gene mutation. The in vitro mammalian cell gene mutation assay is significantly important for detecting point mutations induced by engineered nanomaterials (ENMs) as the bacterial reverse gene mutation assay (Ames test) is not appropriate for use with these materials. | RISKGONE/NILU | doseresponse | Genetic toxicity in vitro | published | |
| DEFAULT | In-vitro assay A generic dose response template. | /IOM | doseresponse | Cell Viability | published | |
| MICRONUCLEUS | Long-Term 3D cytokinesis-blocked micronucleus (CBMN) assay The template for 3D cell models follows the structure of a 2D template. Still, it differs in the “Raw data” spreadsheet, where the metadata expected to be filled up follows the specificity of experiments to detect micronucleus in 3D spheroids (PATROLS, D4.1 SOPs for GIT and liver 3D advanced models optimised for long-term exposures. | PATROLS/SWANSEA | doseresponse | Genetic toxicity in vitro | published | |
| CBMN | Acute 2D/3D cytokinesis-blocked micronucleus (CBMN) assay The micronucleus assay is considered the gold standard for detecting chromosomal damage in vitro. The assay is designed so that cells exposed to a genotoxic test agent result in chromosomal breakage forming small spherical nuclei (micronuclei) being detected as fixed DNA damage. | NANOINFORMATIX/FIOH | doseresponse | Genetic toxicity in vitro | published | |
| BIOIMPEDANCE | Bio impedance - human cells Label-free Cell monitoring by Electrical Impedance (bioimpedance). This method can assess cell viability, proliferation, cell-cell and cell-substrate interaction of adherent cells growing onto a microelectrode array. | RISKGONE/University of Bergen (UiB) | doseresponse | Cell Viability | published | |
| BIOIMPEDANCE_ECOTOX | Bio impedance - ecotoxicity Label-free Cell monitoring by Electrical Impedance (bioimpedance). This method can assess cell viability, proliferation, cell-cell and cell-substrate interaction of adherent cells growing onto a microelectrode array. | RISKGONE/University of Bergen (UiB) | doseresponse | Cell Viability | published | |
| ECOTOX | Ecotoxicity - fish, daphnia, algae, soil (CC BY-SA 4.0) http://dx.doi.org/10.2787/505397 | NANOREG/JRC | ecotox | Short-term toxicity to aquatic inverterbrates | published | |
| ECOTOXTDRF | Ecotoxicity - dose response Dose response template for ecotoxicity experiment | /IOM | doseresponse | Short-term toxicity to aquatic inverterbrates | published | |
| OECD202 | Ecotoxicity: Daphnia magna acute immobilisation test Short-term toxicity to aquatic inverterbrates OECD 202 | /UoB | doseresponse | Short-term toxicity to aquatic inverterbrates | draft | |
| OECD211 | Ecotoxicity: Daphnia magna reproduction test Long-term toxicity to aquatic inverterbrates OECD 211 | /UoB | doseresponse | Long-term toxicity to aquatic inverterbrates | draft | |
| PC_GRANULOMETRY_GRACIOUS | Particle size distribution by ES-DMA, CLS (AUC) (CC BY-SA 4.0) http://dx.doi.org/10.2760/142959 | GRACIOUS/JRC | pchem | Particle size distribution (Granulometry) | published | |
| PC_GRANULOMETRY_NANOREG_RISKGONE | Particle size distribution by TEM, DLS,NTA (RiskGone) (CC BY-SA 4.0) Multiple materials and results in JRC/NANoREG template style - modified by RiskGone http://dx.doi.org/10.2787/505397 | RISKGONE/ | pchem | Particle size distribution (Granulometry) | published | |
| PC_DUSTINESS | Dustiness with small drum (CC BY-SA 4.0) http://dx.doi.org/10.2787/505397 | NANOREG/JRC | pchem | Dustiness | published | |
| DENSITY_HEPYCNOMETER | Density - He Pycnometer (CC BY-SA 4.0) http://dx.doi.org/10.2760/142959 | GRACIOUS/JRC | pchem | Density | published | |
| DENSITY_EFFECTIVE_RISKGONE | Effective density by VCM (RiskGone) (CC BY-SA 4.0) The determination of the effective density of NM by the VCM (Volumetric Centrifugation Method) is based on the measurement of the volume of the pellet obtained by low speed, benchtop centrifugation of NM suspensions in a packed cell volume tube. http://dx.doi.org/10.2760/142959 | RISKGONE/ | pchem | Density | published | |
| ZETAPOTENTIAL_RISKGONE | Zeta Potential by ELS (RiskGone) (CC BY-SA 4.0) The zeta potential is the property defining the surface charge of a material dispersed in a liquid. ELS technique is used to estimate the zeta potential of nanoparticles in suspension from their electrophoretic mobility. The zeta potential and the electrophoretic mobility are then related by the Henry equation. http://dx.doi.org/10.2760/142959 | RISKGONE/ | pchem | Zeta potential | published | |
| PC_SURFACECHEMISTRY_TGA | Surface Chemistry (TGA) (CC BY-SA 4.0) http://dx.doi.org/10.2760/142959 | GRACIOUS/JRC | pchem | Analytical Methods | published | |
| PC_SURFACECHEMISTRY_FTIR | Surface Chemistry (FTIR) (CC BY-SA 4.0) http://dx.doi.org/10.2760/142959 | GRACIOUS/JRC | pchem | Analytical Methods | published | |
| PC_SPECTRA_RAMAN | NANOREG Raman data template (updated by PLASTICFATE) (CC BY-SA 4.0) | PLASTICFATE/CSIC | pchem | Analytical Methods | published | |
| SSA_SEARSTITRATION | Specific Surface Area by Sears Titration (for colloidal silica) (CC BY-SA 4.0) http://dx.doi.org/10.2760/142959 | GRACIOUS/JRC | pchem | Specific surface area | published | |
| HYDROPHOBICITY_CONTACTANGLE | Hydrophobicity by Contact Angle (CC BY-SA 4.0) http://dx.doi.org/10.2760/142959 | GRACIOUS/JRC | pchem | Water solubility | published | |
| SURFACECHEMISTRY_XPS | Surface Chemistry by X-ray Photoelectron Spectroscopy (XPS) (CC BY-SA 4.0) http://dx.doi.org/10.2760/142959 | GRACIOUS/JRC | pchem | Surface chemistry | published | |
| COMPOSITION_XRF | Elemental composition and chemical purity by X-ray Fluorescence (XRF) (CC BY-SA 4.0) http://dx.doi.org/10.2760/142959 | GRACIOUS/JRC | pchem | Analytical Methods | published | |
| CRYSTALLINITY_XRD | Crystallinity by X-Ray diffraction (XRD) (CC BY-SA 4.0) http://dx.doi.org/10.2760/142959 | GRACIOUS/JRC | pchem | Crystalline phase | published | |
| OMICS | Omics metadata (CC BY-NC-SA 4.0) А metadata map for omics data with columns relevant for nanosafety. Omics data stored in e.g. Gene Expression Omnibus (GEO), ArrayExpress, or PRIDE implement omics community standards, including minimum information requirements for omics experiments. However, the databases lack field-specific metadata, such as that defined within the nanosafety community, which leads to poor interoperability with other types of data and databases. | NANOREG2/KI | metadata | Omics metadata | published | |
| OMICSINVIVO | Omics in-vivo metadata (CC BY-NC-SA 4.0) OMICS template extended with fields for in-vivo studies | HARMLESS/KI,NRCWE | metadata | Omics metadata | published |