Data entry templates

IDTemplateProjectProviderTypeCategory
COMET COMET The comet assay, also called SCGE (Single Cell Gel Electrophoresis), is a rapid and informative method to detect DNA damage at single cell level used on many different cell types. The assay detects single and double strand DNA breaks as a consequence of a direct damage or as intermediate of DNA repair processes and it is successfully applied both in in vivo and in vitro genotoxicity testing. RISKGONE NILU doseresponse Genetic toxicity in vitro
CFE Colony Forming Efficiency The colony forming efficiency assay (CFE) (also called clonogenic or plating efficiency assay) measures the ability of cells to survive and form colonies, which is an ultimate index of cytotoxicity. RISKGONE NILU doseresponse Cell Viability
ALAMARBLUE Alamar Blue The Alamar Blue (AB) assay is a high throughput, cell metabolism-based method largely applied in toxicology and nanotoxicology to investigate cell viability (cytotoxicity), cell proliferation and cellular metabolic activity in response to chemicals and nanomaterials. RISKGONE NILU doseresponse Cell Viability
WST1 WST-1 Assay for Cell Proliferation and Viability GRACIOUS BfR doseresponse Cell Viability
NRU Neutral Red Uptake Assay GRACIOUS BfR doseresponse Cell Viability
HPRT HPRT gene mutation assay - SWANSEA layout The hypoxanthine-guanine phosphoribosyl transferase (HPRT) gene is located on the X chromosome of mammalian cells and is used as a model gene to investigate gene mutation. The in vitro mammalian cell gene mutation assay is significantly important for detecting point mutations induced by engineered nanomaterials (ENMs) as the bacterial reverse gene mutation assay (Ames test) is not appropriate for use with these materials. RISKGONE SWANSEA doseresponse Genetic toxicity in vitro
HPRTNILU HPRT gene mutation assay - NILU layout The hypoxanthine-guanine phosphoribosyl transferase (HPRT) gene is located on the X chromosome of mammalian cells and is used as a model gene to investigate gene mutation. The in vitro mammalian cell gene mutation assay is significantly important for detecting point mutations induced by engineered nanomaterials (ENMs) as the bacterial reverse gene mutation assay (Ames test) is not appropriate for use with these materials. RISKGONE NILU doseresponse Genetic toxicity in vitro
DEFAULT In-vitro assay A generic dose response template. IOM doseresponse Cell Viability
DOSERESPONSE_TABLE In-vitro assay (results as a table) A generic dose response template. IOM doseresponse Cell Viability
NEUTROPHIL_SURVIVAL Neutrophil survival Neutrophil survival POLYRISK/UU doseresponse Cell Viability
DCFH DCFH (acellular) GRACIOUS BfR doseresponse Oxidative Stress
CYTOKINES_RELEASE Cytokines release Cytokines release SABYNA RIVM doseresponse Immunotoxicity
ENZYME ACETYLCHOLINESTERASE - INHIBITION, ADSORPTION This type of NM characterisation is called biological characterisation based on enzyme inhibition/adsorption intensity. The more biologically reactive the suspended material (has a potential to inhibit enzyme) or the surface (has a potential to adsorb to the surface) the higher the inhibition of enzyme activity is. Results show that AChE is a promising candidate for ranking different NMs according to their adsorptive and inhibitory properties. PLASTICFATE University of Ljubljana doseresponse Oxidative Stress
MICRONUCLEUS Long-Term 3D cytokinesis-blocked micronucleus (CBMN) assay The template for 3D cell models follows the structure of a 2D template. Still, it differs in the “Raw data” spreadsheet, where the metadata expected to be filled up follows the specificity of experiments to detect micronucleus in 3D spheroids (PATROLS, D4.1 SOPs for GIT and liver 3D advanced models optimised for long-term exposures. PATROLS SWANSEA doseresponse Genetic toxicity in vitro
CBMN Acute 2D/3D cytokinesis-blocked micronucleus (CBMN) assay The micronucleus assay is considered the gold standard for detecting chromosomal damage in vitro. The assay is designed so that cells exposed to a genotoxic test agent result in chromosomal breakage forming small spherical nuclei (micronuclei) being detected as fixed DNA damage. NANOINFORMATIX FIOH doseresponse Genetic toxicity in vitro
BIOIMPEDANCE Bio impedance - human cells Label-free Cell monitoring by Electrical Impedance (bioimpedance). This method can assess cell viability, proliferation, cell-cell and cell-substrate interaction of adherent cells growing onto a microelectrode array. RISKGONE University of Bergen (UiB) doseresponse Cell Viability
BIOIMPEDANCE_ECOTOX Bio impedance - ecotoxicity Label-free Cell monitoring by Electrical Impedance (bioimpedance). This method can assess cell viability, proliferation, cell-cell and cell-substrate interaction of adherent cells growing onto a microelectrode array. RISKGONE University of Bergen (UiB) doseresponse Cell Viability
ECOTOX
(CC BY-SA 4.0)
http://dx.doi.org/10.2787/505397
Ecotoxicity - fish, daphnia, algae, soil NANOREG JRC ecotox Short-term toxicity to aquatic inverterbrates
BIOACCUMULATION_MUSSELS
Bioaccumulation in mussels SBD4NANO INL ecotox Bioaccumulation: aquatic / sediment
OECD236_FET
OECD 236 Fish Embryo Acute Toxicity (FET) Test SBD4NANO INL ecotox Short-term toxicity to fish
ECOTOXTDRF Ecotoxicity - dose response Dose response template for ecotoxicity experiment IOM doseresponse Short-term toxicity to aquatic inverterbrates
OECD202 Ecotoxicity: Daphnia magna acute immobilisation test Short-term toxicity to aquatic inverterbrates OECD 202 UoB doseresponse Short-term toxicity to aquatic inverterbrates
OECD211 Ecotoxicity: Daphnia magna reproduction test Long-term toxicity to aquatic inverterbrates OECD 211 UoB doseresponse Long-term toxicity to aquatic inverterbrates
PC_GRANULOMETRY_GRACIOUS
(CC BY-SA 4.0)
http://dx.doi.org/10.2760/142959
Particle size distribution by ES-DMA, CLS (AUC) GRACIOUS JRC pchem Particle size distribution (Granulometry)
PC_DUSTINESS
(CC BY-SA 4.0)
http://dx.doi.org/10.2787/505397
Dustiness with small drum NANOREG JRC pchem Dustiness
DENSITY_HEPYCNOMETER
(CC BY-SA 4.0)
http://dx.doi.org/10.2760/142959
Density - He Pycnometer GRACIOUS JRC pchem Density
ZETAPOTENTIAL_RISKGONE
(CC BY-SA 4.0)
http://dx.doi.org/10.2760/142959
Zeta Potential by ELS (RiskGone) The zeta potential is the property defining the surface charge of a material dispersed in a liquid. ELS technique is used to estimate the zeta potential of nanoparticles in suspension from their electrophoretic mobility. The zeta potential and the electrophoretic mobility are then related by the Henry equation. RISKGONE pchem Zeta potential
PC_SURFACECHEMISTRY_TGA
(CC BY-SA 4.0)
http://dx.doi.org/10.2760/142959
Surface Chemistry (TGA) GRACIOUS JRC pchem Analytical Methods
PC_TGA_DEGRADATION
Degradation behavior by TGA PLASTICFATE BAM pchem Analytical Methods
PC_PY_GC_MS
Pyrolysis GC-MS for Qualitative Analysis of Polymer Materials Py-GC-MS POLYRISK VU pchem Analytical Methods
AIR_SAMPLING
Air sampling Air sampling POLYRISK UU pchem Analytical Methods
PC_MICROSCOPY_COUNTING
Particle counting by microscopy Microscopy measurements to estimate the number, size distribution and classification of particles POLYRISK Fraunhofer CSP pchem Analytical Methods
PC_VIBRATIONALSPECTROSCOPY_COUNTING
Particle counting by vibrational spectroscopy Vibrational spectroscopy measurements Such as µFT-IR or µRaman are used to estimate the number, size distribution classification and the type of micro and nanoparticles (in special plastics). The analysis of spectra and mapping images can be done using different techniques. POLYRISK Fraunhofer CSP pchem Analytical Methods
PC_SURFACECHEMISTRY_FTIR
(CC BY-SA 4.0)
http://dx.doi.org/10.2760/142959
Surface Chemistry (FTIR) GRACIOUS JRC pchem Analytical Methods
PC_SPECTRA_RAMAN
(CC BY-SA 4.0)
NANOREG Raman data template (updated by PLASTICFATE) PLASTICFATE CSIC pchem Analytical Methods
SSA_SEARSTITRATION
(CC BY-SA 4.0)
http://dx.doi.org/10.2760/142959
Specific Surface Area by Sears Titration (for colloidal silica) GRACIOUS JRC pchem Specific surface area
HYDROPHOBICITY_CONTACTANGLE
(CC BY-SA 4.0)
http://dx.doi.org/10.2760/142959
Hydrophobicity by Contact Angle GRACIOUS JRC pchem Water solubility
SURFACECHEMISTRY_XPS
(CC BY-SA 4.0)
http://dx.doi.org/10.2760/142959
Surface Chemistry by X-ray Photoelectron Spectroscopy (XPS) GRACIOUS JRC pchem Surface chemistry
CRYSTALLINITY_XRD
(CC BY-SA 4.0)
http://dx.doi.org/10.2760/142959
Crystallinity by X-Ray diffraction (XRD) GRACIOUS JRC pchem Crystalline phase
COMPOSITION_XRF
(CC BY-SA 4.0)
http://dx.doi.org/10.2760/142959
Elemental composition and chemical purity by X-ray Fluorescence (XRF) GRACIOUS JRC pchem Analytical Methods
COMPOSITION_ICPMS
(CC BY-SA 4.0)
http://dx.doi.org/10.2760/142959
Elemental composition by ICP-MS GRACIOUS JRC pchem Analytical Methods
COMPOSITION
(CC BY 4.0)
https://doi.org/10.5281/zenodo.7751340
Define material composition Detailed information of material component (chemistry, role, identifiers). HARMLESS IDEA pchem Material composition
OMICS
(CC BY-NC-SA 4.0)
Omics metadata А metadata map for omics data with columns relevant for nanosafety. Omics data stored in e.g. Gene Expression Omnibus (GEO), ArrayExpress, or PRIDE implement omics community standards, including minimum information requirements for omics experiments. However, the databases lack field-specific metadata, such as that defined within the nanosafety community, which leads to poor interoperability with other types of data and databases. NANOREG2 KI metadata Omics metadata
OMICSINVIVO
(CC BY-NC-SA 4.0)
Omics in-vivo metadata OMICS template extended with fields for in-vivo studies HARMLESS KI,NRCWE metadata Omics metadata
PC_GRANULOMETRY_NANOREG
(CC BY-SA 4.0)
http://dx.doi.org/10.2787/505397
Particle size distribution by TEM, DLS NANOREG JRC pchem Particle size distribution (Granulometry)
PC_GRANULOMETRY_DLS
(CC BY-SA 4.0)
http://dx.doi.org/10.2787/505397
Hydrodynamic particle size by DLS NANOREG JRC pchem Particle size distribution (Granulometry)
PC_GRANULOMETRY_SAXS
(CC BY-SA 4.0)
http://dx.doi.org/10.2787/505397
SAXS NANOREG JRC pchem Particle size distribution (Granulometry)
PC_GRANULOMETRY_TEM
(CC BY-SA 4.0)
http://dx.doi.org/10.2787/505397
Particle size distribution by TEM NANOREG JRC pchem Particle size distribution (Granulometry)
PC_GRANULOMETRY_NTA
(CC BY-SA 4.0)
http://dx.doi.org/10.2787/505397
Particle size and particle counting by Nanoparticle Tracking Analysis NTA technique provides size characterization based on the free diffusion behavior of particles in solution. NTA measures particle diffusion by tracking the random motion of single particles in solution via high temporal-resolution video acquisition and enhanced contrast microscopy. The diffusion of the objects in suspension is measured with single-particle resolution, and hence it is particularly suitable for the characterization of size distribution for highly polydisperse nanoparticle populations. NANOREG JRC pchem Particle size distribution (Granulometry)
PC_GRANULOMETRY_NANOREG_RISKGONE
(CC BY-SA 4.0)
http://dx.doi.org/10.2787/505397
Particle size distribution by TEM, DLS,NTA (RiskGone) Multiple materials and results in JRC/NANoREG template style - modified by RiskGone RISKGONE pchem Particle size distribution (Granulometry)
DENSITY_EFFECTIVE
(CC BY-SA 4.0)
http://dx.doi.org/10.2760/142959
Effective density Multiple materials and results in JRC/NANoREG template style GRACIOUS JRC pchem Density
DENSITY_EFFECTIVE_RISKGONE
(CC BY-SA 4.0)
http://dx.doi.org/10.2760/142959
Effective density by VCM (RiskGone) The determination of the effective density of NM by the VCM (Volumetric Centrifugation Method) is based on the measurement of the volume of the pellet obtained by low speed, benchtop centrifugation of NM suspensions in a packed cell volume tube. RISKGONE pchem Density
ZETAPOTENTIAL
(CC BY-SA 4.0)
http://dx.doi.org/10.2760/142959
Zeta Potential GRACIOUS JRC pchem Zeta potential
IL8_ELISA [PATROLS template] ELISA Detection of IL-8 ELISA Detection of IL-8 PATROLS doseresponse Immunotoxicity
TNFA_ELISA [PATROLS template] ELISA Detection of TNF-α ELISA Detection of TNF-α PATROLS doseresponse Immunotoxicity
TEER [PATROLS template] TEER - Transepitelial Electrical Resistance TEER - Transepitelial Electrical Resistance PATROLS doseresponse Cell Viability
PATROLSAB [PATROLS template] Alamar Blue Alamar Blue template by PATROLS PATROLS doseresponse Cell Viability
ECR
(CC BY 4.0)
https://zenodo.org/record/4665253
ECR- Environmental Consumer Release The ECHA Use descriptors will be included in the downloaded template and available for selection in the relevant field. If you don't select any items, all values will be included in the template, otherwise, only the selected items will be included. GRACIOUS LEITAT exposure_release Use and exposure information
ORE
(CC BY 4.0)
https://zenodo.org/record/4665253
ORE- Occupational Release The ECHA Use descriptors will be included in the downloaded template and available for selection in the relevant field. If you don't select any items, all values will be included in the template, otherwise, only the selected items will be included. GRACIOUS LEITAT exposure_release Use and exposure information
DISSOLUTION
(N/A)
Dissolution, dynamic or static GRACIOUS, GOV4NANO and NanoHarmony pchem Water solubility
SSA_BET
(CC BY-SA 4.0)
http://dx.doi.org/10.2760/142959
Specific Surface Area by BET (Brunauer, Emmett and Teller) method GRACIOUS JRC pchem Specific surface area
NRF2ACTIVATION NRF2 Activation assay This template allows data entry for multiple materials. Please specify the number of materials in the relevant web form field. GRACIOUS BfR doseresponse Cell Viability
CARBONYLATION Determining Protein Carbonylation GRACIOUS BfR doseresponse Cell Viability
BARRIERCROSSING Barrier crossing NANOINFORMATIX doseresponse Barrier integrity
REDOXPOTENTIAL Redox Potential NANOINFORMATIX pchem Oxidative Stress
NOPRODUCTION Nitric Oxide production SBD4NANO doseresponse Oxidative Stress
FRAS
(CC-BY 4.0)
https://doi.org/10.5281/zenodo.7729589
Ferric Reduction Ability of Serum Ferric reducing ability of serum (FRAS) assay template. FRAS assay is an indirect assay, based on the principle of absorbance change due to reduction of an Fe3+ complex to Fe2+ complex, by the residual antioxidants in a sample, pre-incubated with a nanomaterial.The absorbance change is correlated to the antioxidant capacity of the serum: the more antioxidants species, the more intense the blue colour. Antioxidants may be suppressed by free radicals produced by nanomaterials, leading to oxidative damage, shown by a reduced blue colour in the assay. HARMLESS BASF doseresponse Oxidative Stress
HEMOLYSIS
(CC-BY 4.0)
?
HEMOLYSIS ? HARMLESS BASF doseresponse Oxidative Stress
DISSOLUTIONIONS
(CC-BY 4.0)
https://doi.org/10.5281/zenodo.7729600
Dynamic dissolution in lung stimulant fluid Dynamic dissolution of a test material in lung simulant fluids is monitored via a continuous flow system consisting of a heat cabinet with reservoir, flow-through cells, peristaltic pump set at 2mL/h and external autosampler. Leaching/biopersistence of relevant metals may be estimated after analysis of the samples in time by a suitable analytical method (i.e., ICP-MS) HARMLESS BASF pchem Water solubility
DISSOLUTIONAQUATIC
(CC-BY 4.0)
https://doi.org/10.5281/zenodo.7729600
Dynamic dissolution (aquatic) Dynamic dissolution (aquatic) HARMLESS BASF pchem Water solubility
MODELS Models metadata Models metadata SBD4NANO Maastricht University metadata Computational models metadata
CHARISMA_RR Raman spectra metadata template Raman spectra metadata including links to spectra files CHARISMA pchem Analytical Methods
HTS_METADATA Metadata including links to data files High Throughput Screening (HTS) metadata, with links to data files HARMLESS metadata Cell Viability
STIS Short term intratracheal instillation STIS HARMLESS NRCWE invivo Acute and sub-chronic toxicity - pulmonary instillation