H2020 CALIBRATE

Performance testing, calibration and implementation of a next generation system-of-systems Risk Governance Framework for nanomaterials

http://www.nanocalibrate.eu/

This project has received funding from the European Union's Horizon 2020 research and innovation programme under grant agreement 686239 caLIBRAte

eNanoMapper database instance

https://apps.ideaconsult.net/calibrate

License: private

nanoreg

MTS CELL VIABILITY ASSAY DAPI Hoechst 33342 Impedance adherent cells ALAMAR BLUE CELL COUNTER Nuclear Intensity Nuclear area CFE Mitochondrial Membrane Potential dye Lysosomal dye Impedance flow cytometry NRU Ki-67 apoptosis cell cycle cleaved caspase-3 Spectrophotometric Method viability COMET COMET FPG RT-PCR MICRONUCLEUS ASSAY Genotoxicity ATM phospho S1981 H2AX phospho S139 p53 phospho s15 CBMN assay Manual scoring ELISA LUMINEX Annexin V_PI (FACS) Griess Reagent Annexin V (Apoptosis) / PI (Necrosis) LUCIFER YELLOW Intestinal barrier integrity intestinal barrier integrity SEM-EDX TEM Reaction Oxygen Species Detection DLS Nanoparticle tracking Analysis SEM supplier WAXS Batch Dispersion Quality VSSA TGA Filtration ICP-MS SOP-Toxicity Test with Daphnia magna (without NOM) Weighing Optical Microscope FACS analysis Colorimetric assay Keylab cell counter CCK8 assay Pig-A gene Swiss roll HE-Pycnometer in vitro oral mucosa 3D model Confocal microscopy TEM/STEM SAXS OECD TG487 MTT assay ELISA MULTIPLEX method barrier test May grunwald giemsa coloration of BAL cells cytospin biochemistry automate ICP-MS automatic count WAXD sp_ICP-MS Aerosol Characterisation XRD Dustiness JRCNM01001a ISO 10872:2010 Water QualityDetermination of the toxic effects of sediment and soil samples on growth, fertility and reproduction of Caenorhabditis elegans OECD TG 202 Acute Toxicity OECD TG 201 ATR-FTIR HAADF-STEM CHN-analysis commercial kits manual count ICP-OES Potentiometry PSEUDOKIRCHNERIELLA SUBCAPITATA CLS Colimetry Propidium iodide Manual count /100 cells vibrofluidization Vortex Shaker LDH DCFH2-DA EDX TRYPAN BLUE PrestoBlue protocol A13261

nanotest

Expression of adhesion molecules radioactive tag labelling DHE DCFH2-DA MTT Assay WST-1 IL6, TNF, etc By ELISA Cytokinesis Block Micronucleus Cytome (CBMN Cyt) COMET NanoTest FeCell Chemical composition of particles flow cytometry LDH Propidium Iodide uptake fluorescent rhodamine-labeled TEM SAED DHE (Dihydroethidium) and Hoechst 33342

enpra

WST-1 LDH ALAMAR BLUE COMET Electron Paramagnetic Resonance (EPR) Fibroblast proliferation

marina

TEER NRU Resazurin LDH CFE Embryonic Stem Cell Test WST-1 ELISA Internalization of latex beads by cells, macrophage activity, inflammation Prothrombotic effects upon systemic administration Differential Centrifugal Sedimentation (DCS) SEM X-ray Photoelectron Spectroscopy (XPS) Electron Paramagnetic Resonance (EPR) TGA ALAMAR BLUE CFDA-AM OECD TG 202 OECD TG 225 OECD TG 217 Plant root length elongation Genotoxicity MICRONUCLEUS ASSAY ICP-MS ICP-OES DLS XRD Porosity OECD TG 210 OECD TG 201 systemic administration

nanogenotox

MICRONUCLEUS ASSAY COMET MLA-TK COMET FPG RICC TCC (Coulter counter) TRYPAN BLUE RN XTT ICP-OES TEM SAXS TGA autopsy analysis blood kinetics analysis EDS DLS BET animal info Flow cytometry with PI staining XRD

calibrate

CellTiter-Glo (CTG) Automated hematology analyzer Short term oral toxicity Wright-Giemsa modified staining & optical microscopy Plasma (Heparin) Jejunum BAL cell celularity Histopathology COMET MICRONUCLEUS ASSAY AO Fluorescence Hyperspectral image analysis Quantitative PCR EPI-200-SIT OCL-200-EIT TEM DLS Supplier Life-history zFET Acridine Orange (AO) n.a.